Kisspeptin-10
10mg
Kisspeptin-10 is the C-terminal decapeptide fragment of the KISS1-derived kisspeptin family and an endogenous ligand for the GPR54 (KISS1R) receptor. Research has focused on its role in the hypothalamic-pituitary-gonadal axis, where it stimulates gonadotropin-releasing hormone signalling, alongside broader investigation of reproductive endocrinology. Supplied as lyophilised powder, third-party tested.
Published research on Kisspeptin-10 is indexed on PubMed. See our full UK buyer's guide for Kisspeptin-10.
Intended Use: Strictly for in-vitro laboratory research. Not for use in humans or animals. Not to be used in foods, drugs, or medical diagnostics. This product has not been evaluated by the MHRA or FDA. Buyer assumes full responsibility for safe handling and regulatory compliance.
≥98%
Purity
LAB
Certified
SAME
Day Ship
Quantity
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Bacteriostatic Water 10ml
Required to reconstitute lyophilised peptides.
Research Use Only
This product is intended strictly for research and laboratory use only. Not for human consumption, medical use, or diagnostic purposes.
Certificate of Analysis
Third-party tested
Lyophilised Stability
24 months
Sealed at -20°C, light-protected
Reconstituted Stability
Up to 3 months
Stored at 2-8°C after mixing
Cold-Chain Shipping
Always
Dry-cold pack, Royal Mail Tracked 24
Important Notice
All compounds are sold individually and do not include research supplies. Products are provided in lyophilised (powder) form and require proper reconstitution before use in research settings.
Related Products
Kisspeptin-10 Price (UK)
Kisspeptin-10 10mg is £34.99 per vial, supplied from stock in the UK with a third-party HPLC certificate published on this page. UK delivery is £4.50 by Royal Mail Tracked 24, or free once your basket reaches £45 (£10.01 away at this price). Orders placed before 2pm on a working day are dispatched the same day.
| Vial | Price | Cost per mg | Availability |
|---|---|---|---|
| Kisspeptin-10 10mgThis page | £34.99 | £3.50 | In stock |
Prices are in GBP and shown per vial as supplied, lyophilised, for in-vitro laboratory research only. Cost per mg is given so researchers can compare vial sizes on a like-for-like basis. Stock and pricing on this table are read live from our inventory, so they match the basket. See our FAQ for delivery and payment options, and quality & testing for how each batch is verified.
Product Specifications
Research Overview
Kisspeptin-10 is the C-terminal decapeptide of human kisspeptin, sequence Tyr-Asn-Trp-Asn-Ser-Phe-Gly-Leu-Arg-Phe-NH₂ (YNWNSFGLRF-NH₂), molecular formula C₆₃H₈₃N₁₇O₁₄, molecular weight 1302.5 g/mol, CAS 374675-21-5. It is also catalogued as kisspeptin-112-121, as metastin (45-54) and as KP-10, and anyone looking to buy kisspeptin should note that kisspeptin-10 and kisspeptin-54 are different molecules processed from the same precursor rather than two names for one compound. The parent peptide was isolated from human placenta by Ohtaki and colleagues (Nature, 2001) as the endogenous ligand of a then-orphan G protein-coupled receptor, and Kotani and colleagues (J Biol Chem, 2001) independently isolated 54-, 14- and 13-residue forms sharing a C-terminal Arg-Phe-amide, placing kisspeptin in the RFamide peptide family. All derive from proteolytic processing of the KISS1 gene product. The receptor, KISS1R and formerly GPR54, is a class A rhodopsin-like G protein-coupled receptor that couples principally through Gq/11 to phospholipase C, with inositol trisphosphate generation, intracellular calcium mobilisation and ERK1/2 phosphorylation as the standard downstream readouts in transfected cell assays. The C-terminal amide is the pharmacophore rather than an incidental modification: structure-activity work by Gutiérrez-Pascual and colleagues (Mol Pharmacol, 2009) mapped which residues of the decapeptide are required for KISS1R activation and examined the solution conformation of the sequence. The receptor's neuroendocrine significance was established by human genetics rather than by pharmacology, when de Roux and colleagues (PNAS, 2003) identified loss-of-function KISS1R mutations in hypogonadotropic hypogonadism, placing the receptor upstream of GnRH neurons in the hypothalamus. Separately, KISS1 was first characterised as a melanoma metastasis-suppressor gene, and KISS1/KISS1R signalling remains an active cancer-biology literature. Supplied as lyophilised powder for in-vitro research use only.
Product Specifications
Laboratory Handling
- Store lyophilised vials at -20°C, protected from light and moisture. Allow the vial to reach room temperature before opening so condensation does not settle on the powder.
- Reconstitute with bacteriostatic water, injecting slowly down the inner glass wall rather than onto the lyophilised cake, then swirl gently until fully dissolved. Never shake or vortex.
- Protect from light throughout handling. The sequence carries a tryptophan at position 3 and two aromatic phenylalanines, and tryptophan oxidation is the degradation route most often picked up as a new RP-HPLC peak in stored aromatic peptides.
- Treat the C-terminal amide as an integrity checkpoint, not a detail. Because amidation of the terminal phenylalanine is required for KISS1R activation, hydrolysis of that amide converts the peptide to a substantially less active free acid one mass unit heavier, which is worth confirming by mass spectrometry after extended storage.
- Hold reconstituted solution at 2-8°C and prepare single-use aliquots at -20°C for longer storage. Avoid repeated freeze-thaw cycles and repeated stopper punctures.
- For cell work note that kisspeptin is reported to be rapidly cleaved by proteases including matrix metalloproteinases, so incubation time and serum content of the medium will both affect the concentration actually present at the receptor.
Frequently Asked Questions
What is Kisspeptin-10?
Kisspeptin-10 is the C-terminal decapeptide of human kisspeptin, sequence Tyr-Asn-Trp-Asn-Ser-Phe-Gly-Leu-Arg-Phe-NH₂ (YNWNSFGLRF-NH₂), molecular formula C₆₃H₈₃N₁₇O₁₄, molecular weight 1302.5 g/mol, CAS 374675-21-5, also catalogued as kisspeptin-112-121, metastin (45-54) or KP-10. It is an agonist at KISS1R, the class A G protein-coupled receptor formerly designated GPR54, which couples principally through Gq/11 and phospholipase C. Kisspeptins belong to the RFamide family, defined by the C-terminal arginine-phenylalanine-amide motif that constitutes the pharmacophore. The parent peptide was isolated from human placenta and reported in Nature in 2001 by Ohtaki and colleagues. Supplied as lyophilised powder for in-vitro research use only, not a licensed medicine.
Kisspeptin-10 vs Kisspeptin-54 (Metastin)
Kisspeptin-10 and kisspeptin-54 are distinct molecules produced by proteolytic processing of the same KISS1 gene product, not two names for one compound. Kotani and colleagues (J Biol Chem, 2001) isolated 54-, 14- and 13-residue peptides from human placenta, all sharing the C-terminal Arg-Phe-amide, while Ohtaki and colleagues (Nature, 2001) reported the 54-residue form, named metastin, as the endogenous ligand of the orphan receptor now called KISS1R. Kisspeptin-10 is the C-terminal ten residues at 1302.5 g/mol, roughly a fifth of the mass of the 54-mer. Both act at the same receptor because the C-terminal decapeptide carries the pharmacophore, but they differ in synthesis cost, solubility, susceptibility to proteolysis and clearance, which is why the shorter form dominates in-vitro receptor pharmacology while the longer form appears more often in whole-animal work. Findings reported for one form should be cited as such and not transferred to the other.
Kisspeptin-10 Research Applications
Kisspeptin-10 is used in vitro as the reference agonist at KISS1R. Typical applications include radioligand binding and competition assays at KISS1R-transfected cell lines, inositol phosphate accumulation and intracellular calcium mobilisation assays reading Gq/11-phospholipase C signalling, ERK1/2 phosphorylation as a downstream readout, receptor desensitisation and internalisation studies, and structure-activity work on RFamide analogues where the decapeptide is the parent scaffold (Gutiérrez-Pascual et al., Mol Pharmacol, 2009). It also appears across two largely separate literatures: hypothalamic neuroendocrinology, where KISS1R expression on GnRH neurons and the loss-of-function mutations reported by de Roux and colleagues (PNAS, 2003) established the receptor's position in the reproductive axis, and tumour biology, where KISS1 was originally characterised as a melanoma metastasis-suppressor gene and KISS1/KISS1R signalling continues to be studied in invasion and metastasis models.
Reconstitution Guide
Allow vials to reach room temperature, then sterilise the rubber stopper of both the peptide vial and the bacteriostatic water vial with an alcohol swab. Slowly draw your bacteriostatic water into a sterile insulin syringe, then inject it gently down the inner glass wall of the peptide vial, never directly onto the lyophilised powder. Once added, gently swirl the vial in a slow circular motion until fully dissolved. Never shake or vortex. Work away from direct sunlight and fluorescent lighting, since the tryptophan at position 3 is the most oxidation-sensitive residue in the sequence and its oxidation is the degradation product most often resolved as a new RP-HPLC peak. The solution should be clear and colourless. Label the vial with the reconstitution date and refrigerate immediately.
See our full peptide reconstitution guide and reconstitution calculator for step-by-step protocol.
Storage Instructions
Lyophilised vials should be stored at -20°C, protected from light and moisture, and remain stable for up to 24 months. Once reconstituted with bacteriostatic water, store at 2-8°C in a light-protected container and use within approximately 4 weeks, preparing single-use aliquots at -20°C for longer storage. Two degradation routes deserve monitoring in this sequence specifically: oxidation of the tryptophan residue, and hydrolysis of the C-terminal phenylalanine amide, which yields a free acid one mass unit heavier and substantially less active at KISS1R because the RFamide motif is the pharmacophore. Mass spectrometry after extended storage is the practical check. Handle under sterile laboratory conditions throughout.
Frequently Asked Questions
Research References
- Metastasis suppressor gene KiSS-1 encodes peptide ligand of a G-protein-coupled receptor (Ohtaki et al., Nature, 2001)
- The metastasis suppressor gene KiSS-1 encodes kisspeptins, the natural ligands of the orphan G protein-coupled receptor GPR54 (Kotani et al., J Biol Chem, 2001)
- Hypogonadotropic hypogonadism due to loss of function of the KiSS1-derived peptide receptor GPR54 (de Roux et al., PNAS, 2003)
- In vivo and in vitro structure-activity relationships and structural conformation of Kisspeptin-10-related peptides (Gutiérrez-Pascual et al., Mol Pharmacol, 2009)
Related Research Compounds
Kisspeptin-10
10mg · £34.99