5-Amino-1MQ
50mg
5-Amino-1MQ (5-amino-1-methylquinolinium) is a small-molecule inhibitor of nicotinamide N-methyltransferase (NNMT). Research has examined NNMT inhibition and its downstream effects on cellular NAD+ salvage, methylation balance and adipocyte energy metabolism, with preclinical work exploring roles in lipid handling and metabolic regulation. Supplied as lyophilised powder, third-party tested.
Published research on 5-Amino-1MQ is indexed on PubMed. See our full UK buyer's guide for 5-Amino-1MQ.
Intended Use: Strictly for in-vitro laboratory research. Not for use in humans or animals. Not to be used in foods, drugs, or medical diagnostics. This product has not been evaluated by the MHRA or FDA. Buyer assumes full responsibility for safe handling and regulatory compliance.
≥98%
Purity
LAB
Certified
SAME
Day Ship
Quantity
Qualifies for free UK delivery · Royal Mail Tracked 24
Damaged or lost? Free reshipment guaranteed
Bacteriostatic Water 10ml
Required to reconstitute lyophilised peptides.
Research Use Only
This product is intended strictly for research and laboratory use only. Not for human consumption, medical use, or diagnostic purposes.
Certificate of Analysis
Third-party tested
Lyophilised Stability
24 months
Sealed at -20°C, light-protected
Reconstituted Stability
Up to 3 months
Stored at 2-8°C after mixing
Cold-Chain Shipping
Always
Dry-cold pack, Royal Mail Tracked 24
Important Notice
All compounds are sold individually and do not include research supplies. Products are provided in lyophilised (powder) form and require proper reconstitution before use in research settings.
Related Products
5-Amino-1MQ Price (UK)
5-Amino-1MQ 50mg is £54.99 per vial, supplied from stock in the UK with a third-party HPLC certificate published on this page. This vial clears the £45 threshold on its own, so UK Royal Mail Tracked 24 delivery is free. Orders placed before 2pm on a working day are dispatched the same day.
| Vial | Price | Cost per mg | Availability |
|---|---|---|---|
| 5-Amino-1MQ 50mgThis page | £54.99 | £1.10 | Low stock |
Prices are in GBP and shown per vial as supplied, lyophilised, for in-vitro laboratory research only. Cost per mg is given so researchers can compare vial sizes on a like-for-like basis. Stock and pricing on this table are read live from our inventory, so they match the basket. See our FAQ for delivery and payment options, and quality & testing for how each batch is verified.
Product Specifications
Research Overview
5-Amino-1MQ is a small molecule, not a peptide, and getting that right is the first step in reading the literature: searches for 5 amino 1mq peptide return a compound that is chemically a quaternary quinolinium salt. Its full name is 5-amino-1-methylquinolinium. The active species is the cation C₁₀H₁₁N₂⁺ at 159.21 g/mol, and it is supplied as the iodide salt, C₁₀H₁₁IN₂, molecular weight 286.11 g/mol, CAS 42464-96-0. Its molecular target is nicotinamide N-methyltransferase (NNMT), a cytosolic S-adenosyl-L-methionine-dependent methyltransferase that converts nicotinamide to 1-methylnicotinamide, consuming SAM and releasing S-adenosylhomocysteine. Because nicotinamide would otherwise re-enter the NAD+ salvage pathway through NAMPT and NMN, NNMT activity sits at a junction between cellular methylation capacity and NAD+ regeneration, which is why the enzyme became a target after Kraus and colleagues (Nature, 2014) reported the consequences of antisense Nnmt knockdown in white adipose tissue and liver. 5-Amino-1MQ is a substrate-mimetic inhibitor that occupies the nicotinamide binding site, with a reported IC₅₀ of 1.2 ± 0.1 μM (Neelakantan et al., J Med Chem, 2017). Its practical advantage over earlier quinolinium analogues is permeability. Neelakantan and colleagues (Biochem Pharmacol, 2018) reported that 1-methylquinolinium and 1-methylnicotinamide showed no measurable passive diffusion in their assays, whereas 5-amino-1MQ crossed by both passive and active routes with no detectable efflux in Caco-2 cells, and lowered intracellular 1-methylnicotinamide in differentiated 3T3-L1 adipocytes with an EC₅₀ near 2.3 μM. Selectivity was reported against COMT and against the SAM-dependent DNA and protein methyltransferases DNMT1 and PRMT3. Human evidence is absent: every in-vivo study published to date is rodent, and no NNMT inhibitor is approved anywhere. Supplied for in-vitro research use only.
Product Specifications
Laboratory Handling
- Store the dry solid at -20°C, sealed and protected from light and moisture. Allow the vial to reach room temperature before opening so condensation does not settle on the contents.
- Because this is a small-molecule salt rather than a peptide, the usual peptide precautions about mechanical shear and methionine or tryptophan oxidation do not apply. It can be dissolved by gentle inversion; there is no need to avoid agitation for structural reasons.
- Dissolve in bacteriostatic water or the aqueous buffer required by the assay. As a permanently charged quaternary cation it is water-soluble and does not require organic cosolvent for aqueous work, though DMSO stocks are common in cell-based screens.
- Protect solutions from light and store at 2-8°C, preparing single-use aliquots for longer storage at -20°C. Iodide salts are light-sensitive and yellowing of a stored solid or solution warrants re-checking identity before use.
- For NNMT assays remember that the compound competes at the nicotinamide substrate site, so the apparent potency depends on the nicotinamide concentration used. Report substrate and SAM concentrations alongside any IC₅₀ so results can be compared with the published values.
- Handle under standard laboratory chemical hygiene. This is a research chemical supplied for in-vitro use only and is not for human or veterinary administration.
Frequently Asked Questions
What is 5-Amino-1MQ?
5-Amino-1MQ is a small-molecule inhibitor of nicotinamide N-methyltransferase (NNMT), not a peptide. Its full name is 5-amino-1-methylquinolinium; the active species is the quaternary cation C₁₀H₁₁N₂⁺ at 159.21 g/mol and it is supplied as the iodide salt, C₁₀H₁₁IN₂, molecular weight 286.11 g/mol, CAS 42464-96-0. NNMT is a cytosolic S-adenosyl-L-methionine-dependent methyltransferase that converts nicotinamide to 1-methylnicotinamide, spending SAM and diverting nicotinamide away from the NAD+ salvage pathway. 5-Amino-1MQ is a substrate-mimetic inhibitor occupying the nicotinamide binding site, with a reported IC₅₀ of 1.2 ± 0.1 μM (Neelakantan et al., J Med Chem, 2017). It is supplied as a research chemical for in-vitro laboratory use only and is not a licensed medicine.
5-Amino-1MQ vs NAD+ Precursors (NAD+, NMN, NR)
These approach the same pathway from opposite directions. NAD+, NMN and nicotinamide riboside supply the salvage pathway with material, adding substrate at or near the top of the route to the dinucleotide. 5-Amino-1MQ instead removes a drain on that route: by inhibiting NNMT it prevents nicotinamide from being methylated to 1-methylnicotinamide, leaving it available for NAMPT and the salvage pathway, and simultaneously spares the S-adenosyl-L-methionine that the methylation would have consumed. The two strategies are therefore not substitutes, and they have very different evidence profiles. The precursor literature includes human pharmacokinetic work; the NNMT inhibitor literature does not, with every published in-vivo study of 5-Amino-1MQ conducted in rodents and no NNMT inhibitor approved in any jurisdiction. They also differ chemically, which matters at the bench: 5-Amino-1MQ is a permanently charged quaternary quinolinium salt, so molar calculations, solubility and analytical identity follow small-molecule rather than nucleotide or peptide conventions.
5-Amino-1MQ Research Applications
5-Amino-1MQ is used as a selective chemical probe for NNMT in enzymology and cell-based metabolic research. Reported applications include NNMT enzyme inhibition assays measuring 1-methylnicotinamide formation or S-adenosylhomocysteine release, intracellular target-engagement assays in differentiated 3T3-L1 adipocytes where 5-Amino-1MQ reduced intracellular 1-methylnicotinamide with an EC₅₀ near 2.3 μM (Neelakantan et al., Biochem Pharmacol, 2018), membrane permeability and Caco-2 transport characterisation, methyltransferase selectivity panels covering COMT, DNMT1 and PRMT3, and rodent studies of NNMT inhibition in adipose tissue and liver following the antisense knockdown work of Kraus and colleagues (Nature, 2014). Because it competes at the nicotinamide substrate site rather than the SAM cofactor site, it is also used as the substrate-competitive reference compound against which bisubstrate and covalent NNMT inhibitors are benchmarked.
Reconstitution Guide
5-Amino-1MQ is a small-molecule salt rather than a peptide, so the peptide handling rules about shear and gentle swirling exist for aseptic reasons only, not structural ones. Allow the vial to reach room temperature, sterilise the stopper of both the compound vial and the bacteriostatic water vial with an alcohol swab, then draw the diluent into a sterile syringe and add it to the vial. As a permanently charged quaternary cation the compound is water-soluble and requires no organic cosolvent for aqueous work, though DMSO stock solutions are common in cell-based screening. Invert or swirl until fully dissolved and inspect for undissolved solid. Label the vial with the preparation date and the concentration, noting whether the concentration refers to the iodide salt at 286.11 g/mol or to the free cation at 159.21 g/mol, since that choice changes every downstream molar calculation.
See our full peptide reconstitution guide and reconstitution calculator for step-by-step protocol.
Storage Instructions
Store the dry solid at -20°C, sealed and protected from light and moisture, where it remains stable for extended periods. Once in solution, store at 2-8°C protected from light and prepare single-use aliquots at -20°C for longer storage. Iodide salts are light-sensitive, so keep stocks in amber or foil-wrapped vessels and re-check identity if a stored solid or solution develops a yellow tint. Freeze-thaw sensitivity is lower than for a peptide, since there is no secondary structure to lose, but aliquoting still avoids repeated stopper punctures and concentration drift from evaporation. Handle under standard laboratory chemical hygiene, for in-vitro research use only.
Frequently Asked Questions
Research References
- Structure-Activity Relationship for Small Molecule Inhibitors of Nicotinamide N-Methyltransferase (Neelakantan et al., J Med Chem, 2017)
- Selective and membrane-permeable small molecule inhibitors of nicotinamide N-methyltransferase (Neelakantan et al., Biochem Pharmacol, 2018)
- Nicotinamide N-methyltransferase knockdown protects against diet-induced obesity (Kraus et al., Nature, 2014)
Related Research Compounds
5-Amino-1MQ
50mg · £54.99