Metabolic Research
NAD+
500mg
Cellular redox coenzyme. Mitochondrial and sirtuin pathway research compound.
Third-party tested · Batch UKPL-6235 · View certificate
- Same-day dispatch before 3pm, Mon to Fri
- Add £14.05 more for free UK delivery
- Cold-chain shipping. Always. Dry-cold pack, Royal Mail Tracked 24
- Lost, damaged or wrong? Replaced free if you tell us within 48 hours.
- Research use only. For in-vitro laboratory research. Not for use in humans or animals, or in food, drugs or diagnostics. Not evaluated by the MHRA or FDA. The buyer is responsible for handling and regulatory compliance.
Bulk Pricing
Counted across your basket, any mix of products.
| Vials in basket | Discount | Per vial |
|---|---|---|
| 1 | None | £54.95 |
| 3-4 | 5% | £52.20 |
| 5-9 | 8% | £50.55 |
| 10-50 | 12% | £48.36 |
Bacteriostatic Water 10ml
Required to reconstitute lyophilised peptides.
What is NAD+?
NAD+ (nicotinamide adenine dinucleotide, also written NAD plus) is the oxidised form of the cell's main redox cofactor. It is a dinucleotide, not a peptide. We supply it as lyophilised powder in 500mg and 1000mg vials.
Full description
The formula is C₂₁H₂₇N₇O₁₄P₂, the molecular weight 663.43 g/mol and the CAS number 53-84-9. Nicotinamide mononucleotide is joined to adenosine monophosphate through a pyrophosphate bridge. Redox chemistry happens on the nicotinamide ring, which accepts a hydride to give NADH.
Signalling enzymes such as sirtuins and PARPs consume NAD+ as a substrate (Liu et al., Cell Metab, 2018). Cells rebuild it through salvage pathways from nicotinamide, nicotinic acid or nicotinamide riboside. NAMPT catalyses the first step from nicotinamide (Grozio et al., J Biol Chem, 2013).
NAD+ Research Applications
- Signalling: NAD+ activates sirtuins and supports mitochondrial function, including through the mitochondrial unfolded protein response (Cantó, Menzies and Auwerx, Cell Metab, 2015).
- Turnover: isotope tracing in cell lines showed NAD made from nicotinamide and consumed largely by PARPs and sirtuins (Liu et al., 2018).
- CD38: Camacho-Pereira and colleagues (Cell Metab, 2016) identified CD38 as the main enzyme degrading the precursor NMN in vivo.
Study details
Typical in-vitro uses are dehydrogenase assays read at 340 nm, sirtuin, PARP and CD38 activity assays, and NAD+/NADH ratio measurements in bioenergetics work.
NAD+ vs NMN vs NR (Nicotinamide Riboside)
NAD+ is the finished dinucleotide. NMN (nicotinamide mononucleotide) and NR (nicotinamide riboside) are precursors upstream of it in the salvage pathways. At the cell surface, CD38 converts NMN to nicotinamide and CD73 converts it to NR. In human cells, CD73 enabled extracellular NMN to feed NAD+ synthesis inside the cell, and CD38 impaired it (Grozio et al., 2013). For cell-free and enzyme work, NAD+ is the cofactor added directly.
Research Overview
Liu and colleagues (Cell Metab, 2018) measured NAD fluxes with isotope tracers instead of relying on concentrations. In vivo, NAD was made from tryptophan selectively in the liver, which then excreted nicotinamide. Fluxes varied widely between tissues: high in the small intestine and spleen, low in skeletal muscle.
Cantó, Menzies and Auwerx (Cell Metab, 2015) review how NAD+ metabolism links energy status to responses in mitochondria and the nucleus. Grozio and colleagues (2013) showed that low micromolar extracellular NAD+, NMN or NR each reversed tumour cell death caused by the NAMPT inhibitor FK866.
Product Specifications
- Molecular Formula
- C₂₁H₂₇N₇O₁₄P₂
- Molecular Weight
- 663.43 g/mol
- CAS Number
- 53-84-9
- Content
- 609.54 mg verified (121.9% of claim)
- Batch
- UKPL-6235
- Form
- White lyophilised powder
- Content assay
- 609.54 mg verified (500 mg label claim)
Certificate of Analysis
Third-party tested
- Batch
- UKPL-6235
- Purity
- 609.54 mg content (121.9% of claim)
- Method
- Content assay
- Lab
- Janoshik Analytical
- Tested
- Sep 2026
Need the certificate for an earlier batch? Request an older CoA
Storage and Reconstitution
Store NAD+ powder at -20°C, away from light and moisture, for up to 24 months. After reconstitution, keep the solution at 2-8°C and use it within 4 weeks. For longer storage, freeze single-use aliquots at -20°C and avoid repeated freeze-thaw cycles.
Vials are sold on their own, without bacteriostatic water for mixing or other supplies.
How to reconstitute and handle
Let the vial and the bacteriostatic water reach room temperature and swab both stoppers with alcohol. Add the water slowly down the inside wall of the vial and swirl gently until dissolved. Do not shake. Avoid alkaline buffers: NAD+ is fairly stable in acid but hydrolyses in base, at both the nicotinamide-ribose bond and the pyrophosphate link.
- Check integrity by UV: NAD+ absorbs at 260 nm, and only NADH adds the 340 nm band.
- At 663.43 g/mol, 500mg of NAD+ is 0.754 mmol and 1000mg is 1.51 mmol.
For the full method, see the peptide reconstitution guide and the reconstitution calculator.
Research References
- NAD+ Metabolism and the Control of Energy Homeostasis (Cantó et al., Cell Metab, 2015)
- CD38 Dictates Age-Related NAD Decline and Mitochondrial Dysfunction (Camacho-Pereira et al., Cell Metab, 2016)
- Quantitative Analysis of NAD Synthesis-Breakdown Fluxes (Liu et al., Cell Metab, 2018)
- CD73 as a source of extracellular precursors for NAD+ biosynthesis (Grozio et al., J Biol Chem, 2013)
Published research on NAD+ is indexed on PubMed. See our full UK buyer's guide for NAD+.
NAD+ Price (UK)
NAD+ is for sale in 2 vial sizes, priced from £54.95 for 500mg to £84.99 for 1000mg. Every size ships from our UK facility, each with its own batch certificate. UK delivery is £4.50 by Royal Mail Tracked 24, free over £69.
| Vial | Price | Cost per mg | Availability |
|---|---|---|---|
| NAD+ 500mgThis page | £54.95 | £0.11 | In stock |
| NAD+ 1000mg | £84.99 | £0.085Best value | In stock |
Prices are in GBP per vial and match the basket. See the FAQ for delivery and payment options, and quality & testing for how each batch is checked.
Frequently Asked Questions
What is NAD+ (nicotinamide adenine dinucleotide)?
The oxidised form of nicotinamide adenine dinucleotide: C₂₁H₂₇N₇O₁₄P₂, 663.43 g/mol, CAS 53-84-9. It carries hydride for redox enzymes and is a substrate for signalling enzymes such as sirtuins and PARPs.
Is NAD+ the same as NAD plus?
Yes. NAD plus is how NAD+ is written where the superscript charge cannot be typed. The reduced form is NADH.
What is the difference between NAD+ and NADH?
They are the oxidised and reduced forms of one redox couple. NAD+ accepts a hydride on its nicotinamide ring to become NADH. Only NADH absorbs at 340 nm, which is how dehydrogenase reactions are followed.
What is the difference between NAD+, NMN and NR?
NAD+ is the finished cofactor. NMN and NR are precursors upstream of it in the salvage pathways, so cells must convert them before they can work as a cofactor.
Why does NAD+ decline with age in research models?
Increased breakdown is the leading explanation. Camacho-Pereira and colleagues (Cell Metab, 2016) found CD38 expression and activity rise with ageing. CD38 was required for the age-related NAD decline and mitochondrial dysfunction, partly through SIRT3.
Can cells take up NAD+ directly?
The cited work points to processing at the cell surface first. Grozio and colleagues (2013) found CD73 converts extracellular NMN to NR, which human cells then use to build NAD+. CD38 opposes that route.
What is in a NAD+ vial?
NAD+ as white lyophilised powder: 500mg in the standard vial or 1000mg in the larger one. Each size has its own batch and certificate.
Where can I buy NAD+ in the UK?
You can buy NAD+ online from UK Peptide Lab, in 500mg and 1000mg vials from UK stock. Order before 3pm (UK time) on a working day for same-day dispatch. UK delivery is free over £69.
Is your NAD plus 500mg UK third-party tested?
Yes. The 500mg batch has a third-party certificate, published in the certificate section. It is a content assay: it reports the NAD+ found in the vial against the label claim. The 1000mg vial has its own certificate, which reports HPLC purity.
What is NAD+ used for in research?
In-vitro enzymology and bioenergetics: dehydrogenase assays, sirtuin, PARP and CD38 activity, and NAD+/NADH ratio work.
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